P152 The prevalence of Thiopurine-methyltransferase and NUTD15 in patient with ulcerative colitis: a hospital-based study in central Taiwan

Abstract Background Thiopurine S-methyltransferase (TPMT) is a cytosolic enzyme involved in the metabolism of these thiopurine drugs. Methylation drugs by TPMT competes with formation their active 6-thioguanine nucleotide metabolite, thereby potentially modulating therapeutic and toxic effects Polymorphisms Nudix hydrolase-15 (NUDT15) have been implicated as predominant cause thiopurine-induced leukopenia Western East Asian countries, but frequency risk alleles lower Asians. Recently, variant NUDT15 was reported to be associated early severe The exact role polymorphisms patients ulcerative colitis (UC) Taiwan uncertain. aim this study investigate prevalence NUDT 15 variants other characteristics UC central Taiwan. Methods From January 1980 November 2022, total 172 consecutive at China Medical University Hospital, tertiary referral center Taiwan, were enrolled study. Clinical UC, including gender, age diagnosis, disease phenotype behavior, collected. We used polymerase chain reaction-restriction fragment length polymorphism method determine allelic frequencies NUD15 our patients. Results A into current mean diagnostic 46.1 years (ranging from 21 75 old). Male accounted for majority all present (64.5%). Among tested 138 patients, which showed TPMT*1 98.5% (136/138) UC. TPMT*3C 1.5% (2/138) No TPMT*2, 3A, 3B, 3D 4-8 found populations. In addition, 123 normal metabolizer 109 (88.6%, 109/123), intermediate 14 (11.4%, 14/123), poor 0 patient (0%, 0/123). Conclusion mutation rate significantly higher than reached 11.4%. leukopenia. measurement can increase safety dramatically successful example personalized medicine field Phenotypic analysis could useful better manage therapy thiopurine.